ICSI is an acronym for intra-cytoplasmic sperm injection. ICSI is a very effective method to get fertilization of eggs in the IVF lab after they have been retrieved from the female partner. IVF with ICSI involves the use of specialized micromanipulation tools and equipment and inverted microscopes that enable embryologists to select and then pick up individual sperms in a tiny specially designed hollow ICSI needle. Then the needle is carefully advanced through the outer shell of the egg and egg membrane and the sperm is then injected into the inner part (cytoplasm) of the egg. This will usually result in normal fertilization in approximately 70-85% of eggs injected with viable sperm. First, the woman must be stimulated with medications and have an egg retrieval procedure so that we can obtain several eggs in order to attempt in vitro fertilization and ICSI.

Procedure

• The mature egg is held with a specialized holding pipette.
• A very delicate, sharp and hollow needle is used to immobilize and pick up a single sperm.
• This needle is then carefully inserted through the zona (shell of egg) and in to the cytoplasm of the egg.
• The sperm is injected in to the cytoplasm and the needle carefully removed.
• The eggs are checked the next morning for evidence of normal fertilization.

Most IVF programs see that about 70-85% of eggs injected using ICSI become fertilized. Pregnancy success rates for in vitro fertilization procedures with ICSI have been shown in some studies to be higher than for IVF without ICSI. This is because in many of the cases needing ICSI the female is relatively young and fertile (good egg quantity and quality) as compared to some of the women having IVF for reasons other than male factor infertility.

ICSI success rates vary according to the specifics of the individual case, the ICSI technique used, the skill of the individual performing the procedure, the overall quality of the laboratory, the quality of the eggs, and the embryo transfer skills of the infertility specialist physician performing the procedure.

Sometimes IVF with ICSI is done for "egg factor" cases - low ovarian reserve situations. This is when there is either a low number of eggs, or lower "quality" eggs (or often both). In such cases, ICSI fertilization and pregnancy success rates are somewhat lower (as a group) since the main determinant of IVF success is the quality of the transferred embryos - and the quality of the eggs is the most crucial factor determining the quality and viability of the resulting embryo.

• Laser Assisted Hatching
• In Vitro Fertilization (IVF)
• Surgical sperm retrival ...etc

• Jindal Heart Institute & Test Tube Baby Centre
• Jinemed Hospital
• Florence Nightingale Group
• Apollo Fertility and IVF Center
• Jaslok Hospital and Research Centre

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